Abstract: Objective　To observe the effects of electroacupuncture on organ coefficient and ovarian aromatase (CYP19A1) gene and protein expression induced by high-fat diet in female growing rats. Methods　21-day-old female SD rats were randomly divided into control, model and acupuncture groups, with 6 rats in each group. The control group was fed with regular feed; the model group was fed with high-fat feed without any treatment; the electroacupuncture group was fed with high-fat feed and selected “Sanyinjiao” (SP6), “Fenglong” (ST40) and “Zusanli” (ST36) for electroacupuncture treatment for 14 days. The body mass and length of rats were measured weekly. After treatment, the body mass, liver mass, ovarian mass, and uterine mass of rats were weighed on an electronic balance. The liver coefficient (liver mass/body mass), ovarian coefficient (ovarian mass/body mass), and uterine coefficient (uterus mass/body mass) were calculated. Fluorescence quantitative PCR was used to detect the expression level of CYP19A1 mRNA in rat ovaries, Western blot method for detecting the expression level of CYP19A1 protein in rat ovarian tissue.Results　Compared with the control group, the body mass and Lee’s index of the model group were significantly increased (P<0.05), the organ coefficient was significantly increased (P<0.05), and the expression of ovarian CYP19A1 mRNA and protein was significantly increased (P<0.05). Compared with the model group, the body weight and Lee’s index of the acupuncture group rats were significantly reduced (P<0.05), while the organ coefficient and the expression of ovarian CYP19A1 mRNA and protein were significantly downregulated (P<0.05). Conclusion　Electroacupuncture can reduce the Lee’s index and organ coefficient of high-fat diet induced obese female growth period rats, downregulate the expression levels of ovarian CYP19A1 mRNA and protein, and thus regulate the level of sexual development in female growth period obese rats.

Key words: electroacupuncture, nutritional obesity, organ coefficients, aromatase

摘要： 目的　探讨电针对高脂饮食诱导的雌性生长期大鼠脏器系数和卵巢芳香化酶(CYP19A1)基因及蛋白表达的 影响。方法　21日龄SD雌性大鼠,随机分为对照组6只,予普通饲料喂养;模型组6只,予高脂饲料喂养,不做任 何治疗;电针组6只,高脂饲料喂养并选取“三阴交”“丰隆”“足三里”给予电针治疗,连续干预14 d。每周测量大 鼠体质量和体长,治疗结束后,分别采用电子天平称重法测量大鼠体质量、肝质量、卵巢质量和子宫质量,并计算肝 系数(肝质量/体质量)、卵巢系数(卵巢质量/体质量)和子宫系数(子宫质量/体质量);采用荧光定量PCR法检测 大鼠卵巢CYP19A1 mRNA的表达水平;Western blot法检测大鼠卵巢组织CYP19A1蛋白的表达水平。结果　与对 照组比较,模型组体质量、Lee’s指数明显升高(P<0.05),脏器系数明显升高(P<0.05),卵巢CYP19A1 mRNA、蛋白 的表达明显升高(P<0.05);与模型组比较,针刺组大鼠体质量、Lee’s指数明显降低(P<0.05),脏器系数及卵巢 CYP19A1 mRNA、蛋白的表达显著下调 (P<0.05)。结论　电针可以降低高脂饮食诱导的雌性生长期肥胖大鼠的 Lee’s指数和脏器系数,下调卵巢CYP19A1 mRNA及蛋白的表达水平,从而调节雌性生长期肥胖大鼠性发育水平。

关键词: 电针, 营养性肥胖, 脏器系数, 芳香化酶